Journal: Avicenna Journal of Medical Biotechnology

Article Title: An Anti-TAZ Monoclonal Antibody Recognizing Cell Surface Expressed TAZ Protein in Human Tumor Cells

doi: 10.18502/ajmb.v15i1.11420

Figure Lengend Snippet: Reactivity of 1F3 with cell surface TAZ on membranes of Burkitt's lymphoma, breast cancer and squamous cell carcinoma lines using flow cytometry technique. Values demonstrate the percentages of positive cells. 1F3 detected cell surface expression of TAZ in pathological conditions with no reactivity in normal conditions. Anti-TAZ mAb was applied on: A) A431, B) MCF-7, C) Raji D) HFFF-PI6 E) MDA-MB-231 G) Normal PBMC (anti-TAZ mAb) and anti-TAZ pAb was used on F) A431 (commercial pAb anti TAZ).

Article Snippet: To evaluate the localization of TAZ protein, flow cytometry analysis was performed both at intracellular and cell surface staining using both produced anti-TAZ mAb and polyclonal anti-TAZ antibody (Abcam).

Techniques: Flow Cytometry, Expressing

Journal: Bioscience Reports

Article Title: Mitochondrial membrane potential played crucial roles in the accumulation of berberine in HepG2 cells

doi: 10.1042/BSR20190477

Figure Lengend Snippet: ( A ) HepG2 cells were incubated with 30 μM berberine at 37°C for 0.5, 1, 2, 4, or 6 h, respectively. ( B ) HepG2 cells were incubated with 0.9, 3, 9, 30, 90, or 300 μM berberine at 37°C for 0.5 h, respectively. ( C ) HepG2 cells were incubated with 0.9, 3, 9, 30, 90, or 300 μM berberine at 4 or 37°C for 0.5 h, respectively. After incubation, the cells were washed three times with chilled PBS and harvested by trypsinisation. The cells were stored in 0.5 ml chilled PBS and the accumulated berberine in the cells was semi-quantified by flow cytometry analysis with excitation at 488 nm and emission at 530 nm.

Article Snippet: The concentration of berberine and Rho123 (a organic cation used to reflex the MMP in the study) in the cells were semi-quantified by flow cytometry analysis (Becton-Dickenson FACSCaliburTM system, New Jersey, U.S.A.) according to a previous report [ ].

Techniques: Incubation, Flow Cytometry

Journal: Bioscience Reports

Article Title: Mitochondrial membrane potential played crucial roles in the accumulation of berberine in HepG2 cells

doi: 10.1042/BSR20190477

Figure Lengend Snippet: ( A ) HepG2 cells were incubated with HBSS or HBSS-K containing 10 μM berberine at 37°C for 0.5 h, respectively. ( B ) HepG2 cells were incubated with 10 μM berberine or in the presence of cimetidine (0.3, 1 mM, Cim) or rifampicin (10 μM, Rif) at 37°C for 0.5 h, respectively. After incubation, the cells were washed three times with chilled PBS and harvested by trypsinisation. The cells were stored in 0.5 ml chilled PBS and the accumulated berberine in the cells was quantified by flow cytometry analysis with excitation at 488 nm and emission at 530 nm. ** indicates P <0.01 versus control or between 0.3 or 1 mM cimetidine-treated groups.

Article Snippet: The concentration of berberine and Rho123 (a organic cation used to reflex the MMP in the study) in the cells were semi-quantified by flow cytometry analysis (Becton-Dickenson FACSCaliburTM system, New Jersey, U.S.A.) according to a previous report [ ].

Techniques: Incubation, Flow Cytometry, Control

Journal: Bioscience Reports

Article Title: Mitochondrial membrane potential played crucial roles in the accumulation of berberine in HepG2 cells

doi: 10.1042/BSR20190477

Figure Lengend Snippet: HepG2 cells were pre-incubated with drug-free HBSS or HBSS containing 0.125, 0.25, 0.5, 1, or 2 μM CCCP at 37°C for 20 min, then equal volume of HBSS containing 20 μM berberine or 6 μg/ml Rho123 was added and the cells were further incubated at 37°C for 0.5 h. After incubation, the cells were washed three times with chilled PBS and harvested by trypsinisation. The cells were stored in chilled PBS and the concentration of berberine in the cells was quantified by flow cytometry analysis with excitation at 488 nm and emission at 530 nm. * indicates P <0 . 05 while ** indicates P <0.01 versus groups that treated without CCCP.

Article Snippet: The concentration of berberine and Rho123 (a organic cation used to reflex the MMP in the study) in the cells were semi-quantified by flow cytometry analysis (Becton-Dickenson FACSCaliburTM system, New Jersey, U.S.A.) according to a previous report [ ].

Techniques: Incubation, Concentration Assay, Flow Cytometry

Journal: Bioscience Reports

Article Title: Mitochondrial membrane potential played crucial roles in the accumulation of berberine in HepG2 cells

doi: 10.1042/BSR20190477

Figure Lengend Snippet: HepG2 cells were incubated with 10 μM berberine and with or without 0.125 μM CCCP, 0.3 or 1 mM cimetidine (Cim), or the HBSS-K solution 37°C for 0.5 h, respectively. After incubation, the cells were washed three times with chilled PBS and harvested by trypsinisation. The cells were stored in 0.5 ml chilled PBS and the concentration of berberine in the cells was quantified by flow cytometry analysis with excitation at 488 nm and emission at 530 nm. ( A ) Synergistic inhibition of CCCP and HBSS-K on berberine uptake in the HepG2 cells; ( B ) synergistic inhibition of HBSS-K and cimetidine on berberine uptake in the HepG2 cells; ( C ) influence of CCCP on the inhibition of HBSS-K in terms of berberine uptake in the HepG2 cells; ( D ) inhibition of the combination of CCCP, HBSS-K, and cimetidine on berberine uptake in the HepG2 cells.* indicates P <0 . 05 while ** indicates P <0.01 versus control or between groups indicated by the lines.

Article Snippet: The concentration of berberine and Rho123 (a organic cation used to reflex the MMP in the study) in the cells were semi-quantified by flow cytometry analysis (Becton-Dickenson FACSCaliburTM system, New Jersey, U.S.A.) according to a previous report [ ].

Techniques: Incubation, Concentration Assay, Flow Cytometry, Inhibition, Control

Journal: Bioscience Reports

Article Title: Mitochondrial membrane potential played crucial roles in the accumulation of berberine in HepG2 cells

doi: 10.1042/BSR20190477

Figure Lengend Snippet: HepG2 cells were pre-incubated with 10 μM berberine at 37°C for 2 h, then the cells were incubated with drug-free HBSS or HBSS containing CCCP (0.25 μM), or verapamil (100 μM), or CCCP (0.25 μM) plus verapamil (100 μM) at 37°C for 0.5, 1, 2, 4 h, respectively. After incubation, the cells were washed three times with chilled PBS and harvested by trypsinisation. The cells were stored in 0.5 ml chilled PBS and the accumulated berberine in the cells was quantified by flow cytometry analysis with excitation at 488 nm and emission at 530 nm. The amount of berberine was normalised to that at zero time point. * indicates P <0.05 while ** indicates P <0.01 versus verapamil treated groups.

Article Snippet: The concentration of berberine and Rho123 (a organic cation used to reflex the MMP in the study) in the cells were semi-quantified by flow cytometry analysis (Becton-Dickenson FACSCaliburTM system, New Jersey, U.S.A.) according to a previous report [ ].

Techniques: Incubation, Flow Cytometry